HPLC detection of dehydroepiandrosterone in food additives by using normal phase HPLC

hydrochlorothiazide detection in urine samples by hplc-dad and experimental design dispersive l-l microextraction

hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...

Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The...

Analysis of Phospholipids in Natural Samples by Normal Phase HPLC and Corona Charged Aerosol Detection

Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC

The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C(18) column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The re...

The determination of biotin in food using HPLC